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An efficient and reproducible method to culture Bergmann and cortical radial glia using textured PMMA

Autor
Álvarez, Z.; Sena, E.; Mattotti, M.; Engel, E.; Alcantara, S.
Tipus d'activitat
Article en revista
Revista
Journal of neuroscience methods
Data de publicació
2014-07-30
Volum
232
Pàgina inicial
93
Pàgina final
101
DOI
https://doi.org/10.1016/j.jneumeth.2014.05.011 Obrir en finestra nova
Repositori
http://hdl.handle.net/2117/24863 Obrir en finestra nova
URL
http://www.sciencedirect.com/science/article/pii/S0165027014001654# Obrir en finestra nova
Resum
Radial glia cells comprise the principal population of neural stem cells (NSC) during development. Attempts to develop reproducible radial glia and NSC culture methods have met with variable results, yielding non-adherent cultures or requiring the addition of growth factors. Recent studies demonstrated that a 2-µm patterned poly-methyl methacrylate (ln2 PMMA) grooved scaffold, by mimicking the biophysical and microtopographic properties of the embryonic NSC niche, induces the de-differentiation...
Citació
Álvarez, Z. [et al.]. An efficient and reproducible method to culture Bergmann and cortical radial glia using textured PMMA. "Journal of neuroscience methods", 30 Juliol 2014, vol. 232, p. 93-101.
Paraules clau
Astrocytes, Radial Glia, Bergmann Glia, Progenitors, Poly-methyl Methacrylate (pmma), Micro-patterning, Surface Topography
Grup de recerca
BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
CREB - Centre de Recerca en Enginyeria Biomedica

Participants

  • Álvarez Pinto, Zaída  (autor)
  • Sena, Elena  (autor)
  • Mattotti, Marta  (autor)
  • Engel Lopez, Elisabet  (autor)
  • Alcantara, Soledad  (autor)