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Interpulse multifrequency electrical impedance measurements during electroporation of adherent differentiated myotubes

Autor
García-Sánchez, T.; Azan, A.; Leray, I.; Rosell, F.; Bragos, R.; Mir, Lluís M.
Tipus d'activitat
Article en revista
Revista
Bioelectrochemistry
Data de publicació
2015-10-01
Volum
105
Pàgina inicial
123
Pàgina final
135
DOI
https://doi.org/10.1016/j.bioelechem.2015.05.018 Obrir en finestra nova
Repositori
http://hdl.handle.net/2117/78849 Obrir en finestra nova
URL
http://www.ncbi.nlm.nih.gov/pubmed/26123676 Obrir en finestra nova
Resum
In this study, electrical impedance spectroscopy measurements are performed during electroporation of monolayers of differentiated myotubes. The time resolution of the system (1 spectrum/ms) enable 860 full spectra (21 frequencies from 5 kHz to 13 MHz) to be acquired during the time gap between consecutive pulses (interpulse) of a classical electroporation treatment (8 pulses, 100 mu s, 1 Hz). Additionally, the characteristics of the custom microelectrode assembly used allow the experiments to b...
Citació
García-Sánchez, T., Azan, A., Leray, I., Rosell, F., Bragos, R., Mir, Lluís M. Interpulse multifrequency electrical impedance measurements during electroporation of adherent differentiated myotubes. "Bioelectrochemistry", 01 Octubre 2015, vol. 105, p. 123-135.
Paraules clau
Adherent cells, Cole model, Electrical impedance spectroscopy, Electrical model, Electroporation, Gene-transfer, High-voltage pulses, In-vivo, Irreversible electroporation, Mammalian-cells, Micro-electroporation, Monitoring electropermeabilization, Patch-clamp, Single-cell electroporation, Tissue electroporation
Grup de recerca
CREB - Centre de Recerca en Enginyeria Biomedica
IEB - Instrumentació Electrònica i Biomèdica

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