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  • EWLP 2014 - 13th European Workshop on Lignocellulosics and Pulp

     Cusola Aumedes, Oriol; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
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  • Removal of lignin and hexenuronic acids from sisal fibers with novel xylanases belonging to different GH families

     Valenzuela, Susana V.; Valls Vidal, Cristina; Roncero Vivero, Maria Blanca; Vidal Llucia, Teresa; Diaz, Pilar; Pastor Blasco, Francisco Ignacio Javier
    European Workshop on Lignocellulosics and Pulp
    p. 839-842
    Presentation's date: 2014-06-27
    Presentation of work at congresses

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    The aim of this study was to evaluate five different preparations of xylanases from Paenibacillus barcinonensis, a highly xylanolytic species, who is able to grow on xylan as a sole carbon source. As raw material to assess, fibers from sisal (Agave sisalana) were chosen. Sisal pulps were bleached by an XP sequence, where X denotes the enzyme treatment and P a hydrogen peroxide extraction stage. Kappa number, brightness, viscosity and hexenuronic acid content of samples were determined. Sugars released from sisal pulps, other non-wood fibres and also eucalyptus fibres, by the treatment with the xylanases were also analysed. The best results were obtained with the GH10 xylanase and with crude supernatants of P. barcinonensis, which produced a lignin removal of 23% and a reduction of 25% in the hexenuronic acid content of sisal pulps without a significant loss of viscosity. The xylanases of family GH30 showed good performance, being more efficient when applied as a single catalytic domain. On the contrary, Xyn11E did not show any significant effect on pulp properties. The release of sugars in the effluents from the X stage applied to sisal correlated with the effectiveness of the xylanases tested. The xylan content of wood and non-wood fibres, the type of xylan and its accessibility also had an influence on the xylanase activity on pulps

  • Using glycosidases to modify TCF bleached specialty sisal fibers

     Beltramino Heffes, Facundo; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    European Workshop on Lignocellulosics and Pulp
    p. 211-214
    Presentation's date: 2014-06-25
    Presentation of work at congresses

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    Modifying fibers in order to upgrade them to dissolving grade involves several changes. Paper grade pulps can be used to yield this kind of raw materials, if the necessary modifications are carried out. Classic processes are capable of producing these changes. However, these classic processes present many drawbacks. In this direction biotechnology, and particularly enzymes, are attracting an increasing interest due to the special features they present. Enzymes (glycosidases) are applied in this work on an alkaline TCF bleached sisal pulp. A xylanase and a cellulase preparation, both commercial, are applied on this pulp in order to evaluate their potential as catalysts for carrying out the previously stated upgrade. The main objective is to remove hemicelluloses present in starting pulp, which are an undesirable impurity in final product. Other modifications are as well profitable, such as increases in cellulose reactivity, a key parameter in dissolving pulps quality. After application, it is observed that final pulps present a lower content in hemicelluloses, a higher reactivity as well as other positive modifications

  • Functionalization of cellulosic model substrates via laccase-mediated coupling of non-polar particles

     Cusola Aumedes, Oriol; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca; Rojas, Orlando J.
    European Workshop on Lignocellulosics and Pulp
    p. 119-122
    Presentation's date: 2014-06-27
    Presentation of work at congresses

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    In the present work we investigate the physicochemical interactions between silica and nanofibrillar cellulose (CNF) with a multi component system (MCS) obtained from an enzymatic reaction of a laccase enzyme and a short-chain organic molecule, dodecyl 3,4,5-trihydroxybenzoate (commonly known as lauryl gallate, LG) as well as sulphonated lignin (SL). Hydrophobic chains of enzyme-modified LG were coupled onto CNF and silica surfaces by direct adsorption of the MCS. Quartz crystal microgravimetry (QCM-D), Atomic Force Microscopy (AFM) and water contact angle (WCA) were used to monitor in situ and characterize the hydrophobization process. Efficient adsorption of the MCS onto CNF and silica surfaces increased their WCA by 88° and 78°, respectively. Dynamic Light Scattering (DLS) measurements revealed an effect of the enzyme on LG: reducing particle size from several microns down to 300 nm. The laccase (Lacc) treatment in the presence of SL reduced even more the LG particle size to 80 nm through a dispersive effect of SL

  • Exploring biobleaching possibilities of dissolving pulps by means of enzymatic treatments

     Quintana Vilajuana, Elisabet; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    European Workshop on Lignocellulosics and Pulp
    p. 695-698
    Presentation's date: 2014-06-27
    Presentation of work at congresses

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    Unbleached sulphite cellulose was subjected to an LQPo biobleaching sequence with the intention to elucidate the potential of LMS as an alternative to conventional bleaching processes. The enzymatic stage (L) was performed with the presence of Trametes villosa laccase and violuric acid. This enzymatic stage was followed by a chelating stage (Q) and then by a hydrogen peroxide stage reinforced with pressurized oxygen (Po). The outstanding results obtained with laccase-violuric acid system fulfil the characteristics of commercial dissolving-grade pulp (high reactivity, high ISO brightness, preserved cellulose integrity and low content of hemicellulose). Additionally, the enzymatic treatment saved 2h of reaction time and about 70% of hydrogen peroxide consumption, relative to a conventional hydrogen peroxide sequence (Po).

    Unbleached sulphite cellulose was subjected to an LQPo biobleaching sequence with the intention to elucidate the potential of LMS as an alternative to conventional bleaching processes. The enzymatic stage (L) was performed with the presence of Trametes villosa laccase and violuric acid. This enzymatic stage was followed by a chelating stage (Q) and then by a hydrogen peroxide stage reinforced with pressurized oxygen (Po). The outstanding results obtained with laccase-violuric acid system fulfil the characteristics of commercial dissolving-grade pulp (high reactivity, high ISO brightness, preserved cellulose integrity and low content of hemicellulose). Additionally, the enzymatic treatment saved 2h of reaction time and about 70% of hydrogen peroxide consumption, relative to a conventional hydrogen peroxide sequence (Po)

  • Evaluating changes on cellulosic biobleached fibers by means of thermogravimetric analysis

     Quintana Vilajuana, Elisabet; Garcia Barneto, Agustín; Valls Vidal, Cristina; Vidal Llucia, Teresa; Ariza, J.; Roncero Vivero, Maria Blanca
    European Workshop on Lignocellulosics and Pulp
    p. 691-694
    Presentation's date: 2014-06-27
    Presentation of work at congresses

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    The effects on fibre surface produced by the laccase-mediator system on softwood sulphite fibres were analysed in terms of surface crystallinity using thermogravimetric analysis. The starting pulp presented low content of lignin and hemicelluloses and these characteristics were clearly reflected on TGA graphs, indicating a clean and crystalline surface. Unbleached sulphite pulps were subjected to a biobleaching process using the well-know laccase-mediator system (LMS). From all studied mediators, violuric acid, VA, was the most efficient biobleaching compound. By contrast, HBT also provided a good delignification but affected negatively the fibre ¿surface¿. The natural mediators, SA and PCA were not grafted on sulphite pulp unlike what have been observed with other raw materials. These observations led to the development of an extended biobleaching sequence named LVAQPo, using a laccase from Trametes villosa in combination with VA as a mediator. The characterization of treated pulp in terms of dissolving pulp characteristics showed that this enzymatic sequence could satisfy the market-like requirements. In addition, TGA results showed that the introduction of an enzymatic stage let to reduce the adverse effect caused by a hydrogen peroxide treatment in terms of pulp crystallinity

  • Evaluating the impact of combining mediators in the LMS for biobleaching or functionalization

     Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    European Workshop on Lignocellulosics and Pulp
    p. 843-846
    Presentation's date: 2014-06-27
    Presentation of work at congresses

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    In this work, a laccase from Cerrena Unicolor (CuL) supplied by Fungal Bioproducts® was firstly applied in combination with VA in Eucalyptus globulus in order to compare its biobleaching effect with the well-known laccase from Trametes villosa supplied by Novozymes®. However, the main purpose of this work was to apply the CuL in presence of two different mediators like VA (as synthetic compound) or p-coumaric acid (PCA), acetosyringone (AS) or syringaldehide (SA) (as natural compounds). Each compound was applied alone or in combination with another one in order to find a possible biobleaching or functionalising boosting effect between them. Concerning the biobleaching effects, the highest delignification and brightness increase was produced with the VA applied alone. The natural mediators failed to increase delignification and contrary, they hindered the effect of VA due to possible interactions with the natural phenols and fibres. Concerning the grafting effects, the highest increase in kappa number and in colour properties was produced with the combination of PCA and AS. Finally, the best way of applying these mediators, together in one step (LAS+PCA), or in two steps with (LASwashing-LPCA) or without (LAS-LPCA) washing was evaluated

  • Coating of laccase-activated phenols for the industrially-feasible functionalization of cellulose-based substrates

     Cusola Aumedes, Oriol; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    European Workshop on Lignocellulosics and Pulp
    p. 295-298
    Presentation's date: 2014-06-25
    Presentation of work at congresses

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    The present work describes an innovative method for the hydrophobization of cellulosic material by impregnation with an enzymatically obtained functionalization solution (FS). Application of FS to the surface of previously formed cellulose sheets was found to confer them hydrophobic properties. The absorbance of functionalized sheets was assessed with the water-drop test (WDT), and their hydrophobicity from their contact angle (WCA) and the surface free energy (SFE) as determined with a goniophotometer. The proposed method is an effective choice for the hydrophobization of paper sheets, with absorption times of up to 4000 s and WCA values around 130°. Treating eucalyptus paper sheets dramatically decreased SFE (from 55 to 10 mJ/m2), and evidences on the grafting of the FS onto the cellulosic sheets were assessed by using ATR-FTIR. The stability of FS and the control solutions was characterized in terms of Z potential and light scattering measurements

  • Valortec

     Cusola Aumedes, Oriol; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
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  • Surface functionalization of cellulosic substrates by using chemical and biotechnological methods.

     Cusola Aumedes, Oriol
    Universitat Politècnica de Catalunya
    Theses

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    La present tesi tracta sobre la modificació superficial de substrats cel·lulòsics utilitzant mètodes químics i enzimàtics. Lafuncionalització de la cel¿lulosa consisteix en ajustar-ne les seves propietats, per a poder-la utilitzar en gran varietatd'aplicacions. Habitualment aquesta modificació s'aconsegueix tractant les fibres en suspensió aquosa i abans de laformació del substrat. No obstant, en la present tesi els tractaments s'apliquen utilitzant substrats cel¿lulòsics ja formats(FCS), obtenint una sèrie d'avantatges en termes de formació, consum de químics, i velocitat de fabricació. S¿han estudiatdos enfocaments diferents per a la funcionalització: tècniques no-enzimàtiques i enzimàtiques. Els esforços s¿han centrat enl'enfoc enzimàtic.Aquest treball es va dur a terme en el grup de recerca CELBIOTECH (UPC-BarcelonTech), en el marc dels projectesFUNCICEL (CTQ2009-12904) , BIOSURFACEL (CTQ2012-34109) , BIOFIBRECELL (CTQ2010-20238-CO3-01), del MICINNEspanyol i el Projecte integrat BIORENEW (NMP2-CT-2006-026456) del sisè Programa Marc.Pel que fa a la funcionalització no-enzimàtica, s¿han utilitzat -ciclodextrines (-CD) per a la modificació superficial de tresFCS comercials. L'empelt s¿ha avaluat mitjançant microscòpia electrònica de rastreig i anàlisi FTIR. Els substrats empeltatsamb -CDs s¿han carregat amb digluconat de clorhexidina (digCHX), i s¿ha analitzat l¿alliberament d¿aquest principi actiuutilitzant espectroscòpia UV. Diversos substrats han estat capaços de retenir quantitats significatives de digCHX, i demantenir-ne l¿alliberament durant períodes de temps de fins a 20 dies.La funcionalització enzimàtica per al desenvolupament de derivats de la cel·lulosa ha guanyat interès en la comunitatcientífica i industrial darrerament. No obstant, l'ús de sistemes enzimàtics superficials encara es troba en una fase moltprematura; la present tesi fa èmfasi en aquest aspecte. En un primer estudi s¿analitza la hidrofobització superficial de FCScomercials, mitjançant una lacasa en combinació amb compostos hidròfobs. L'eficiència s¿ha vist augmentada amb l'ús delignosulfonats (SL), els quals milloren la distribució superficial, augmenten els nivells d¿hidrofobicitat, i ajuden a preservarl'activitat enzimàtica. La dosi de LG i el temps de tractament s¿han optimitzat resultant en una major hidrofobicitat.Seguidament, els tractaments han evolucionat cap a un nou mètode basat en l¿obtenció d'una solució funcionalitzadora (FS),consistent en un producte derivat d¿una reacció enzimàtica, el qual és aplicat posteriorment a la superfície dels FCS. Aquestmètode també s¿ha mostrat efectiu, i proporciona avantatges importants respecte a la possible aplicació industrial; de fet, elnou mètode ha permès realitzar una sol·licitud internacional de patent. L¿aplicació de la FS a la superfície de FCS elsconfereix propietats hidrofòbica i antioxidant gràcies a les interaccions fisicoquímiques que es produeixen. S¿han utilitzatsuperfícies model de cel·lulosa per tal d¿avaluar l'adsorció de la FS, i s¿han emprat les tècniques de WDT, WCA, SEM, AFM iSFE per caracteritzar-les. La força de l¿enllaç s¿avalua mitjançant rentats agressius i extraccions Soxhlet. Es demostra perprimer cop el potencial dels tractaments enzimàtics superficials per a conferir propietats avançades als substrats cel·lulòsics. La tècnica també s¿ha assajat emprant diverses lacases.S¿han utilitzat diverses tècniques d¿anàlisi per estudiar els mecanismes de reacció, i les interaccions FS-substrat. Lestècniques han estat: DLS, QCM, FTIR, espectroscopia UV-VIS, potencial-Z, Turbiscan® i Voltametria Cíclica. S¿ha proposatl¿estructura química de les molècules resultants de l¿oxidació del LG, els possibles mecanismes d'oxidació, i possiblesmecanismes d'empelt entre els compostos modificats i la cel·lulosa. També s¿ha analitzat l'efecte de la longitud de lacadena alquílica i dels tractaments tèrmics en les propietats antioxidant i hidrofòbica

  • ESTUDIO Y DESARROLLO PROCEDIMIENTOS PARA FUNCIONALIZACION SUPERFICIAL DE SOPORTES LIGNOCELULOSICOS MEDIANTE SISTEMAS ENZIMATICOS

     Galea Martinez, Silvia; Andreu Terren, Maria Gloria; Valls Vidal, Cristina; Roncero Vivero, Maria Blanca
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  • Enzymatically treated sulphite pulp to give dissolving-pulp characteristics

     Quintana Vilajuana, Elisabet; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    International Symposium on Wood, Fiber and Pulping Chemistry
    Presentation's date: 2013
    Presentation of work at congresses

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    Bleached cellulose with good end-properties (~90% ISO brightness, < 0.5 kappa number and substantially preserved cellulose integrity) was obtained by using a totally chlorine-free (TCF) biobleaching sequence. The initial pulp was subjected to an overall LQPo sequence. Unbleached sulphite cellulose was treated with Trametes villosa laccase (L) in combination with violuric acid (VA). This enzymatic stage was followed by a chelating stage (Q) and then by a hydrogen peroxide stage reinforced with pressurized oxygen (Po). The use of violuric acid was dictated by the results of a preliminary study, where the bleaching efficiency of various natural (syringaldehyde and pcoumaric acid) and synthetic mediators (violuric acid and 1-hydroxybenzotriazole) were assessed. The outstanding results obtained with laccase-violuric acid system fulfil the characteristics of commercial dissolving-grade pulp with the added advantage that the enzymatic treatment saved 2h of reaction time and about 70% of hydrogen peroxide consumption, relative to a conventional hydrogen peroxide sequence (Po)

  • Functionalization of cellulosic substrates using a product derived from an enzymatic reaction

     Cusola Aumedes, Oriol; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    International Symposium on Wood, Fiber and Pulping Chemistry
    Presentation's date: 2013
    Presentation of work at congresses

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    The present work describes a new method for the surface functionalization of cellulosic sheets, overcoming the limitations of the traditional treatments, which are usually performed using fibrous suspensions. The method consists in obtaining a product (post-enzymatic) derived from an enzymatic reaction using Laccase and a compound showing a specific property. The obtained product, which has aqueous consistency, is then applied to the surface of a finished paper sheet (or cellulosic substrate) by means of impregnation, spray, size-press, or other available surface deposition technique used in the industry. The functionalization is produced by means of physicochemical interaction between the product and the substrate. In the present research, the methodology is developed to achieve the hydrophobization of finished paper sheets using Lauryl Gallate (LG) as hydrophobic compound, and a laccase derived from Trametes Villosa, reaching absorption times of up to 4000 s and CA values around 130° as assessed by the water drop test (WDT) and contact angle measurements (CA) respectively.

    The present work describes a new method for the surface functionalization of cellulosic sheets, overcoming the limitations of the traditional treatments, which are usually performed using fibrous suspensions. The method consists in obtaining a product (post-enzymatic) derived from an enzymatic reaction using Laccase and a compound showing a specific property. The obtained product, which has aqueous consistency, is then applied to the surface of a finished paper sheet (or cellulosic substrate) by means of impregnation, spray, size-press, or other available surface deposition technique used in the industry. The functionalization is produced by means of physicochemical interaction between the product and the substrate. In the present research, the methodology is developed to achieve the hydrophobization of finished paper sheets using Lauryl Gallate (LG) as hydrophobic compound, and a laccase derived from Trametes Villosa, reaching absorption times of up to 4000 s and CA values around 130° as assessed by the water drop test (WDT) and contact angle measurements (CA) respectively

  • A new use for TCF pulp with a high hexenuronic acid content

     Valls Vidal, Cristina; Roncero Vivero, Maria Blanca
    International Symposium on Wood, Fiber and Pulping Chemistry
    Presentation's date: 2013
    Presentation of work at congresses

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    The importance of HexA groups lies on their influence on the bleaching process and the properties of the resulting pulp. In this work, various aspects of HexA were examined in order to elucidate their role in eucalyptus fibres. Thus, the contribution of HexA to kappa number in eucalyptus pulp was estimated to be 1.05 units per 10 µmol of HexA. Also, HexA changes during an ECF and a TCF sequence were examined in relation to lignin. Interesting results were found by comparing the boosting effect of xylanase and the impact of the alkaline stage in both sequences. The antioxidant activity of eucalyptus pulp with a variable HexA content was also measured. Although the presence of HexA in pulp has so far been deemed detrimental, HexA can have a favourable effect derived from their antioxidant ability. This property of HexA can be useful to obtain TCF paper for use in food packaging for easily oxidized products

    The importance of HexA groups lies on their influence on the bleaching process and the properties of the resulting pulp. In this work, various aspects of HexA were examined in order to elucidate their role in eucalyptus fibres. Thus, the contribution of HexA to kappa number in eucalyptus pulp was estimated to be 1.05 units per 10 μmol of HexA. Also, HexA changes during an ECF and a TCF sequence were examined in relation to lignin. Interesting results were found by comparing the boosting effect of xylanase and the impact of the alkaline stage in both sequences. The antioxidant activity of eucalyptus pulp with a variable HexA content was also measured. Although the presence of HexA in pulp has so far been deemed detrimental, HexA can have a favourable effect derived from their antioxidant ability. This property of HexA can be useful to obtain TCF paper for use in food packaging for easily oxidized products

  • High cellulose-content alkaline pulps obtained by enzyme-assisted hemicelluloses removal

     Beltramino Heffes, Facundo; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    International Symposium on Wood, Fiber and Pulping Chemistry
    Presentation's date: 2013
    Presentation of work at congresses

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    Several procedures have been studied in order to carry out the necessary modifications to ¿upgrade¿ paper-grade pulps to dissolving grade including various chemical and biotechnological procedures. Among the last ones, enzymes, and particularly xylanases have a special interest due to their specificity characteristics. In the present work, xylanases are applied on a bleached alkaline pulp (lignin content close to 1%) in order to eliminate xylans. To achieve the highest purity grade, it is desirable to remove as much hemicelluloses as possible. Xylanases are applied on a TCF bleached nonwood NaOH-AQ pulp from Sisal (Agave Sisalana) at different conditions (xylanase dose, reaction time and procedure). The aim of this research is to find the best conditions for this application and establish an enzymatic procedure for obtaining pulps with low xylans content from bleached alkaline pulps. And as a consequence, obtain high cellulose-content fibers with longer durability and improved characteristics

  • Blanqueo de pastas al sulfito mediante tratamientos enzimáticos con lacasa

     Quintana Vilajuana, Elisabet; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    Reunión de la Red Temática Española Lignocel
    Presentation's date: 2012-11
    Presentation of work at congresses

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  • Funcionalización de sustratos celulósicos mediante un producto derivado de una reacción enzimática

     Cusola Aumedes, Oriol; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    Reunión de la Red Temática Española Lignocel
    DOI: BIO2011-15394-E
    Presentation's date: 2012-11-14
    Presentation of work at congresses

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  • Paper functionalization by means of enzymatic surface treatments using hydrophobic compounds and lignosulfonates

     Cusola Aumedes, Oriol; Garcia Ubasart, Jordi; Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    European Workshop on Lignocellulosics and Pulp
    p. 146-149
    Presentation's date: 2012-08-30
    Presentation of work at congresses

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    In the present work an innovative method for achieving enzymatic surface functionalization on finished paper sheets is presented. A commercial filter paper was surface sized with laccase derived from Trametes Villosa, Lauryl Gallate (LG) as hydrophobic compound, and soluble lignin. Moreover, this study shows the positive influence of sulfonated lignin (SL) acting as a dispersant improving the surface distribution of the LG along the paper sheet. The hydrophobic behavior of obtained sheets is comparable to the commercial ones. Hydrophobicity was measured by the water absorption test, and the variation in the contact angle through time was monitorized by a contact angle goniometer. Hydrophobized paper absorption was compared with Teflon® in order to determine the evaporated and absorbed water amounts from the paper surface. The treatment time, was also studied

    Postprint (author’s final draft)

  • Bleached dissolving pulps applying laccase treatments

     Quintana Vilajuana, Elisabet; Valls Vidal, Cristina; Roncero Vivero, Maria Blanca
    European Workshop on Lignocellulosics and Pulp
    p. 464-467
    Presentation's date: 2012-08-27
    Presentation of work at congresses

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    A biobleaching sequence, using a laccase enzyme (Trametes Villosa) in combination with different mediators, was applied to softwood dissolving cellulose in order to study its bleaching efficiency and its potential in terms of kappa number, ISO brightness and viscosity. The tested mediators were classified as synthetic compounds such as HBT (1-hydroxybenzotriazole) and VA (violuric acid), and as natural compounds such as SA (syringaldehyde) and pCA (p-coumaric acid). The influence of the enzymatic stage in the bleaching sequence was compared with a control treatment and also with a conventional alkaline hydrogen peroxide stage. The contribution of L (laccasemediator) stage on the effluent properties is important for evaluating and minimizing its environmental impact. Therefore, effluent properties (COD, colour, residual enzyme activity and toxicity) were determined. Laccase-VA system showed the most promising results in reducing kappa number and increasing brightness without detriment to viscosity

  • BIO2011-15394-E. Biotecnología de Materiales Lignocelulósicos. Retos enzimáticos, químicos y moleculares para su aplicación industrial, energética y medioambiental

     Vidal Llucia, Teresa; Camarero, Susana; Roncero Vivero, Maria Blanca; Valls Vidal, Cristina; Cusola Aumedes, Oriol; Quintana Vilajuana, Elisabet; Beltramino Heffes, Facundo
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  • Preparación enzimática acuosa aislada y su uso para la funcionalización de la superficie del papel o soportes celulósicos

     Cusola Aumedes, Oriol; Roncero Vivero, Maria Blanca; Valls Vidal, Cristina; Vidal Llucia, Teresa
    Date of request: 2012-06-04
    Invention patent

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    Preparación enzimática acuosa aislada y uso para la funcionalización de la superficie del papel o soportes celulósicos.

    En un primer aspecto, la presente invención se refiere a una preparación enzimática acuosa aislada obtenida a partir de la reacción de por lo menos una enzima oxidorreductasa, preferiblemente una lacasa, y por lo menos un producto natural o sintético, preferiblemente un compuesto natural, cuyo producto natural o sintético comprende en su estructura por lo menos un grupo fenol o alcohol, que opcionalmente tiene una o más cadenas hidrofóbicas, o por lo menos un grupo esterol.

    En un segundo aspecto, la presente invención se refiere a la utilización de dicha preparación enzimática acuosa aislada en la funcionalización de la superficie del papel o soportes celulósicos.

  • La química i la societat

     Grau Vilalta, Maria Dolors; Font Soldevila, Jose; Sanz Balague, Joaquim; Guaus Guerrero, Ester; Calvet Tarragona, Aurelio; Salán Ballesteros, Mª Núria; Martínez Martínez, María R.; Farran Marsa, Adriana; Gorchs Altarriba, Roser; Alvarez Del Castillo, M. Dolores; Garrido Soriano, Nuria; Morillo Cazorla, Margarita; Almajano Pablos, Maria Pilar; Cardona Planes, Anna Maria; Macanás de Benito, Jorge; Bonsfills Pedros, Ana; Gamisans Noguera, Javier; Dorado Castaño, Antonio David; Lao Luque, Concepcion; Torrent Burgues, Juan; Molins Duran, Gemma; Colom Fajula, Xavier; Carrillo Navarrete, Fernando; Ramon Portés, Eva; Garriga Sole, Pere; Sole Sardans, M. Montserrat; Ardanuy Raso, Monica; Pares Sabates, Ferran; Roncero Vivero, Maria Blanca; Cusola Aumedes, Oriol
    Collaboration in exhibitions

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  • Study of the effects on bast and core flax fibres of laccase + mediator/phenol treatments

     Fillat Latorre, Amanda; Roncero Vivero, Maria Blanca; Vidal Llucia, Teresa
    Italian Meeting on Lignocellulosic Chemistry
    p. 327-330
    Presentation's date: 2011-09-05
    Presentation of work at congresses

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  • Tratamientos enzimáticos superficiales utilizando lacasa, compuestos hidrófobos y surfactantes

     Cusola Aumedes, Oriol; Roncero Vivero, Maria Blanca; Garcia Ubasart, Jordi; Valls Vidal, Cristina
    Reunión de la Red Temática
    Presentation's date: 2011-09-06
    Presentation of work at congresses

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  • Flax fibre modification using enzyme systems to obtain high-value cellulose products  Open access  awarded activity

     Fillat Latorre, Amanda
    Department of Textile and Paper Engineering, Universitat Politècnica de Catalunya
    Theses

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    The aim of this thesis is to modify flax pulp fibres (Linum usitatissimum) by more friendly environmental processes. Pulp and paper research is focussing through enzyme systems investigation for developing green chemistry technologies due to existing environmental concerns and to legal restrictions. Moreover, it exists also an increasing strategic interest in using flax fibres to obtain high-quality specialty papers. That is why we study the application of biotechnology as an efficient alternative to traditional industrial processes based on the use of chemical agents. This work is framed by two of the main research topics of the Paper and Graphic Specialty Laboratory in the Textile and Paper Engineering Department of the Universitat Politècnica de Catalunya. One research line is based on pulp bleaching and is focused basically on the study of enzymatic systems as biobleaching agents; the other research topic that has been recently introduced in our investigation group is the use of enzymes as functionalisation agents by promoting the grafting of several compounds. Laccase is the main enzyme used in this thesis; it is an oxidoreductase that can assist reactions in an eco-friendly way since laccase uses air and produces water as the only by-product. Moreover, laccase can work under mill conditions and has wide application potential. The first part of this thesis involved the use of enzymes to bleach flax pulp. The aim was to explore the potential of various natural mediators (lignin-derived compounds) for delignifying flax fibres in order to identify the most efficient and ecofriendly choice among them. Afterwards, we assessed the use of various enzyme delignification stages in an industrial bleaching sequence. The ensuing totally chlorine free (TCF) sequence comprised various laccase-mediator system treatments (L stage) followed by a by a chelating stage (Q stage) and a subsequent bleaching step with hydrogen peroxide (Po stage). A xylanase pretreatment was additionally carried out. Laccases used came from the fungi Pycnoporus cinnabarinus and Myceliophthora thermophila; the performance of several natural mediators was compared with the obtained with the application of various synthetic mediators. In addition, the lack of studies on the properties of effluents from the treatment of non-wood pulp with laccase and natural mediators led A-1 A-2 us to examine effluent properties upon biotreatments and after different bleaching stages. The results obtained warrant upscaling any of the biobleaching sequences for flax pulp as they provide sustainable flax fibre with a high cellulose content and brightness above 80% ISO. The use of xylanase pretreatment was found to efficiently remove HexA and enhance delignification by laccase.

  • Enzymatic treatments of paper surface using laccase, hydrophobic compounds and lignin

     Cusola Aumedes, Oriol; Roncero Vivero, Maria Blanca; Garcia Ubasart, Jordi; Valls Vidal, Cristina
    International Colloquium on Eucalyptus Pulp
    Presentation's date: 2011-05-12
    Presentation of work at congresses

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  • A new approach to the internal sizing of paper via biotechnological processes

     Garcia Ubasart, Jordi; Roncero Vivero, Maria Blanca; Vidal Llucia, Teresa
    International Colloquium on Eucalyptus Pulp
    Presentation's date: 2011-05-10
    Presentation of work at congresses

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    This work deals with the study of a new biotechnological procedure, employing laccase in combination with hydrophobic compounds, for the biomodification of fibres and the internal sizing of paper. Cellulose fibres from eucalyptus kraft pulp, unbleached and TCF bleached, were incubated with laccase, in combination with a series of hydrophobes. Laccase treatments of cellulose fibres with lauryl gallate conferred the lowest level of water penetration in handsheets. It was observed that the hydrophobization of paper was not possible using the hidrophobe alone. The coupling of lauryl gallate onto cellulose fibres was firstly denoted by kappa number analysis, and further demonstrated by IR spectroscopy. The internal sizing of paper proved to be effective regardless the lignin content of eucalyptus fibres

  • Nuevas perspectivas para el sistema lacasa-mediador en la industria papelera

     Garcia Ubasart, Jordi; Roncero Vivero, Maria Blanca; Colom, Josep F.; Vidal Llucia, Teresa
    Congreso de Microbiología Industrial y Biotecnología Microbiana
    Presentation's date: 2010-11-17
    Presentation of work at congresses

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  • Oxidative enzymes for bleaching a non-wood pulp

     Fillat Latorre, Amanda; Roncero Vivero, Maria Blanca; Vidal Llucia, Teresa
    Congreso de Microbiología Industrial y Biotecnología Microbiana
    p. 131
    Presentation's date: 2010-11-17
    Presentation of work at congresses

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  • Using biotechnology for paper functionalisation: flax fibers with antimicrobial properties

     Fillat Latorre, Amanda; Vidal Llucia, Teresa; Pastor, F.I.J.; Díaz, Pilar; Roncero Vivero, Maria Blanca; Gallardo Román, Óscar
    TECNICELPA Conference and Exhibition
    Presentation's date: 2010-10-12
    Presentation of work at congresses

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    Unbleached flax fibres were treated with laccase from Pycnoporus cinnabarinus and low molecular weight phenols (syringaldehyde –SA, acetosyringone –AS- and p-coumaric acid –PCA-) to biomodify lignocellulosic fibres in order to confer them antibacterial properties. After the enzymatic treatment, PCA, SA and AS produced an increase in kappa number (lignin estimation), which is due to a partial covalent binding of the phenoxy radicals on pulp. To take advantage of the tendency of these natural phenols to couple to fibres, a standard method to determine bacterial growing inhibition was carried out. Antimicrobial properties of the pulps were tested on S. aureus, P. aeruginosa and K. pneumonia. Treated pulps produced a greater reduction in viable organisms than the laccase control after the same contact time, in some cases nearly total organism elimination was seen. Optical properties of treated papers were also measured in order to evaluate the possible coloration obtained. These results suggest that these low molecular weight phenols are covalently bounded to the flax fibres by the laccase and can act as antimicrobial agents.

  • Assessment of effluents from laccase bleaching treatments

     Valls Vidal, Cristina; Vidal Llucia, Teresa; Quintana Vilajuana, Elisabet; Roncero Vivero, Maria Blanca
    Encontro Nacional de Tecnicelpa
    p. 383-
    Presentation's date: 2010-10-12
    Presentation of work at congresses

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  • Enzymatic treatments with laccase: an alternative to the conventional internal sizing of paper?

     Garcia Ubasart, Jordi; Roncero Vivero, Maria Blanca; Colom Pastor, Josep Francesc; Vidal Llucia, Teresa
    Encontro Nacional de Tecnicelpa
    p. 85
    Presentation's date: 2010-10-12
    Presentation of work at congresses

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  • Proteomics tools for the detection of novel enzymes with application in paper industry

     Valenzuela Mayorga, Susana Valeria; Valls Vidal, Cristina; Roncero Vivero, Maria Blanca; Díaz, Pilar; Pastor Blasco, F.I. Javier
    Encontro Nacional de Tecnicelpa
    p. 388-
    Presentation's date: 2010-10-12
    Presentation of work at congresses

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  • Laccases in paper manufacture: grafting vs. removing aromatic compounds ?

     Vidal Llucia, Teresa; Aracri, Elisabetta; Cadena Chamorro, Edith Marleny; Fillat Latorre, Amanda; Garcia Ubasart, Jordi; Valls Vidal, Cristina; Roncero Vivero, Maria Blanca; Colom Pastor, Josep Francesc
    Oxidative Enzymes as Sustainable Industrial Biocatalysts
    p. 140-144
    Presentation's date: 2010-09-15
    Presentation of work at congresses

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  • Comparing new biobleaching sequences of flax pulps

     Fillat Latorre, Amanda; Roncero Vivero, Maria Blanca; Vidal Llucia, Teresa
    Oxidative Enzymes as Sustainable Industrial Biocatalysts
    p. 205-209
    Presentation's date: 2010-09-14
    Presentation of work at congresses

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  • An innovative method for the internal sizing of paper

     Garcia Ubasart, Jordi; Roncero Vivero, Maria Blanca; Colom Pastor, Josep Francesc; Vidal Llucia, Teresa
    Oxidative Enzymes as Sustainable Industrial Biocatalysts
    p. 182-187
    Presentation's date: 2010-09-14
    Presentation of work at congresses

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  • Advances in eucalypt pulp bleaching: new laccase-mediator treatments

     Valls Vidal, Cristina; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca
    Oxidative Enzymes as Sustainable Industrial Biocatalysts
    p. 214-217
    Presentation's date: 2010-09-14
    Presentation of work at congresses

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  • Characterization of a GH5 xylanase with activity on linear oligosaccharides: Evaluation as a bleaching aid

     Gallardo Román, Óscar; Valls Vidal, Cristina; Valenzuela Mayorga, Susana Valeria; Vidal Llucia, Teresa; Roncero Vivero, Maria Blanca; Díaz, Pilar; Pastor Blasco, F.I. Javier
    COST FP0602: Biotechnical Processing of Lignocellulosic Raw Materials
    p. 22-
    Presentation's date: 2010-09-22
    Presentation of work at congresses

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  • Herramientas proteómicas para la detección de nuevas xilanasas con aplicación en tecnología papelera

     Valenzuela, S.V.; Díaz, P.; Valls Vidal, Cristina; Roncero Vivero, Maria Blanca; Pastor, F.I.J.
    Reunión de la Red Temática
    p. 18
    Presentation's date: 2010-07-12
    Presentation of work at congresses

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  • Aplicación del sistema lacasa-mediador en los procesos de fabricación de papel

     Garcia Ubasart, Jordi; Roncero Vivero, Maria Blanca; Colom Pastor, Josep Francesc; Vidal Llucia, Teresa
    Reunión de la Red Temática
    p. 14
    Presentation's date: 2010-07-12
    Presentation of work at congresses

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  • Flax fiber functionalisation with laccase: obtaining antimicrobial paper

     Fillat Latorre, Amanda; Vidal Llucia, Teresa; Gallardo Román, Óscar; Pastor, F.I.J.; Díaz, P.; Roncero Vivero, Maria Blanca
    Symposium on Biotechnology Applied to Lignocelluloses
    p. 46
    Presentation's date: 2010-03-31
    Presentation of work at congresses

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  • FUNCIONALIZACION DE FIBRAS LIGNOCELULOSICAS MEDIANTE SISTEMAS LACASA MEDIADOR UTILIZANDO COMPUESTOS

     Colom Pastor, Josep Francesc; Vila Babarro, Carlos; Andreu Terren, Maria Gloria; Cadena Chamorro, Edith Marleny; Valls Vidal, Cristina; Aracri, Elisabetta; Galea Martinez, Silvia; Roncero Vivero, Maria Blanca
    Competitive project

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  • UPC Contribution: WP1, SWP4.1; SWP 4.2; WP7, WP8

     Colom Pastor, Josep Francesc; Roncero Vivero, Maria Blanca; Valls Vidal, Cristina; Fillat Latorre, Amanda; Aracri, Elisabetta; Garcia Ubasart, Jordi; Cadena Chamorro, Edith Marleny; Vidal Llucia, Teresa
    Date: 2009-09-22
    Report

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  • Enzymatic delignification

     Colom Pastor, Josep Francesc; Valls Vidal, Cristina; Roncero Vivero, Maria Blanca; Vidal Llucia, Teresa
    Date: 2009-09-22
    Report

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  • How is the pulp sterol content affected by the application conditions of the laccase-mediator treatment?

     Valls Vidal, Cristina; Molina, S.; Vidal Llucia, Teresa; del Rio Andrade, José Carlos; Colom Pastor, Josep Francesc; Martínez, A.T.; Gutiérrez, A.; Roncero Vivero, Maria Blanca
    International Conference on Textile and Polymer Biotechnology
    p. 344-345
    Presentation's date: 2009-09-24
    Presentation of work at congresses

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  • Functionalisation of flax fibres with laccase and natural mediators: development of antimicrobial properties

     Fillat Latorre, Amanda; Gallardo Román, Óscar; Vidal Llucia, Teresa; Pastor Blasco, Francisco Ignacio Javier; Díaz, Pilar; Colom Pastor, Josep Francesc; Roncero Vivero, Maria Blanca
    Annual Workshop of COST FP0602
    p. 23
    Presentation's date: 2009-09-03
    Presentation of work at congresses

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  • PROCEDIMIENTO DE FABRICACIÓN DE PAPEL CON ENCOLADO INTERNO MEDIANTE UN SISTEMA ENZIMA-MEDIADOR

     Colom Pastor, Josep Francesc; Garcia Ubasart, Jordi; Roncero Vivero, Maria Blanca; Vidal Llucia, Teresa
    Date of request: 2009-07-23
    Invention patent

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    Procedimiento de fabricación de papel con encolado interno mediante un sistema enzima-mediador.

    La presente invención se refiere a un procedimiento para la fabricación de papel con encolado interno que comprende las etapas de: a) preparación de fibras celulósicas; b) tratamiento de las fibras obtenidas en la etapa a) con un sistema enzima-mediador que consiste en una enzima oxidativa tipo lacasa y un mediador que es un producto natural o sintético que comprende en su estructura un grupo fenólico o un alcohol, y c) fabricación del papel con las fibras tratadas.

  • Desarrollo de pastas de lino antimicrobianas: funcionalización con el sistema lacasa-mediadores naturales

     Gallardo Román, Óscar; Fillat Latorre, Amanda; Vidal Llucia, Teresa; Pastor Blasco, Francisco Ignacio Javier; Díaz, Pilar; Colom Pastor, Josep Francesc; Roncero Vivero, Maria Blanca
    Reunión de la Red Temática
    p. 6
    Presentation's date: 2009-06-04
    Presentation of work at congresses

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  • Evaluación del NHA para el sistema lacasa-mediador

     Valls Vidal, Cristina; Colom Pastor, Josep Francesc; Baffert, Carole; Gimbert, Isabelle; Roncero Vivero, Maria Blanca; Sigoillot, Jean-Claude
    Reunión de la Red Temática
    p. 7
    Presentation's date: 2009-06-04
    Presentation of work at congresses

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  • Influence of operational conditions of laccase-mediator system on sterols removal

     Valls Vidal, Cristina; Molina, S; Vidal Llucia, Teresa; del Rio Andrade, José Carlos; Colom Pastor, Josep Francesc; Martínez, A.T.; Gutiérrez, A.; Roncero Vivero, Maria Blanca
    International Symposium on Wood, Fiber and Pulping Chemistry
    p. 741-742
    Presentation's date: 2009-06-16
    Presentation of work at congresses

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