In addition to surgery, local tumor control in pediatric oncology requires new treatments as an alternative to radiotherapy. SN-38 is an anticancer drug with proved activity against several pediatric solid tumors including neuroblastoma, rhabdomyosarcoma and Ewing sarcoma. Taking advantage of the extremely low aqueous solubility of SN-38, we have developed a novel drug delivery system (DDS) consisting of matrices made of poly(lactic acid) electrospun polymer nanofibers loaded with SN-38 microcrystals for local release in difficult-to-treat pediatric solid tumors. To model the clinical scenario, we conducted extensive preclinical experiments to characterize the biodistribution of the released SN-38 using microdialysis sampling in vivo. We observed that the drug achieves high concentrations in the virtual space of the surgical bed and penetrates a maximum distance of 2 mm within the tumor bulk. Subsequently, we developed a model of subtotal tumor resection in clinically relevant pediatric patient-derived xenografts and used such models to provide evidence of the activity of the SN-38 DDS to inhibit tumor regrowth. We propose that this novel DDS could represent a potential future strategy to avoid harmful radiation therapy as a primary tumor control together with surgery
Gelatin fibers were prepared by electrospinning of gelatin/acetic acid/water ternary mixtures with the aim of studying the feasibility of fabricating gelatin nanofiber mats at room temperature using an alternative benign solvent by significantly reducing the acetic acid concentration. The results showed that gelatin nanofibers can be optimally electrospun with low acetic acid concentration (25% v/v) combined with gelatin concentrations higher than 300 mg/ml. Both gelatin solutions and electrospun gelatin mats (prepared with different acetic acid aqueous solutions) were analyzed by FTIR and DSC techniques in order to determine the chemical and structure changes
of the polymer. The electrospun gelatin mats fabricated from solutions with low acetic acid content showed some advantages as the maintenance of the decomposition temperature of the pure gelatin (~230ºC) and the reduction of the acid content on electrospun mats, which allowed to reach a cell viability upper than 90% (analyzed by
cell viability test using human dermal fibroblast and embryonic kidney cells). This study has also analyzed the influence of gelatin and acetic acid concentration both on the solution viscosity and the electrospun fiber diameter, obtaining a clear relationship between these parameters
El sistema comprende: - unos medios de dispensación que incluyen una aguja (1) por la punta de la cual dispensar, por electrohilado, una substancia en solución (P), - un colector (3) para recibir la substancia dispensada por la aguja (1) una vez solidificada formando unas fibras (Pt), y - unos medios de suministro de disolvente que comprenden un contenedor (2) de disolvente en estado líquido (Ls) dispuesto cerca de la punta de la aguja (1), para suministrar disolvente en estado gaseoso (Gs) al entorno de salida de la misma, incluyendo la zona del cono de Taylor (Ct), mediante la evaporación del disolvente en estado líquido (Ls). El método comprende suministrar disolvente en estado gaseoso (Gs) al entorno de salida de la punta de la aguja (1), mediante la evaporación localizada, en las proximidades de la zona de creación del cono de Taylor (Ct), de un disolvente en estado líquido (Ls).
Ventura, H.; Ardanuy, M.; Capdevila, F.; Cano, F.; Tornero, J. Journal of the Textile Institute Vol. 105, num. 10, p. 1065-1075 DOI: 10.1080/00405000.2013.874628 Date of publication: 2014-06-26 Journal article
The main objective of this paper is to study the effects of the processing parameters of the needle-punching machine and the interactions between them on some physico-mechanical properties of interest of nonwoven (NW) fabrics. For this purpose, a fractional factorial design has been planned with two levels for each factor: feeding speed, delivery speed, stroke frequency, penetration depth and gap between plates. Sixteen NW fabrics were obtained from polyester fibre and characterized by tensile strength and stiffness for mechanical behaviour and air permeability as physical properties of interest in some technical applications. The results have been subjected to statistical analysis in order to find the effects of the processing variables and the interactions between them on the fabric properties. It was possible to find the effects of the five processing parameters studied on the thickness, fabric mass, stiffness, tensile strength and air permeability of the obtained needle-punched fabrics
The development of suitable biomimetic scaffolds is a fundamental requirement of tissue engineering. Although electrospinning has emerged as an effective method for producing such scaffolds of nanometer-sized fibers, the influence of solution characteristics on the morphology of the resulting nanofibers depends on each polymer solution system. In this study, gelatin nanofibers and microfibers were prepared via electrospinning using mixtures of water and acetic acid at different ratios as solvents. The viscosities of gelatin solutions before electrospinning were analyzed and two different behaviors were found as a function of the solvent composition, taking into account classic models of polymer science. A power law relationship between viscosity and gelatin concentration was found for each solvent system, and an empirical model including the influence of acetic acid was obtained for aqueous systems. Moreover, a ternary diagram considering gelatin, water, and acetic acid mass fractions was constructed as a tool to establish the electrospinnability domains in terms of fiber occurrence and morphology. Also, the isodiametric curves were defined in the fibers region. Finally, in order to correlate the diameter of electrospun nanofibers and the electrospinnability zones, the Berry number was used. However, as its only allows the range of electrospinnability to be established for a fixed solvent composition, a new dimensionless parameter (Bemod) was suggested to take into account all the acetic acid aqueous solutions as a single solvent
Sistema y procedimiento de infusión de resina por vacío para la obtención de piezas elaboradas a partir de materiales compuestos, así como la pieza obtenida mediante dicho procedimiento, comprendiendo el sistema un par de diafragmas flexibles y vinculables entre sí tal que se define un espacio entre ambos diafragmas flexibles para la disposición de una preforma; comprendiendo adicionalmente unos medios de encapsulamiento dispuestos entre los diafragmas flexibles, en el que los medios de encapsulamiento comprenden una bolsa de vacío configurada para albergar una preforma, y unos medios de moldeo posicionados por debajo del diafragma flexible inferior o por encima del diafragma flexible superior y que comprenden un cuerpo de molde sobre una superficie de vacío, estando adaptada dicha superficie de vacío tal que se genera una cavidad sellada cuando se vincula a alguno de los diafragmas flexibles.
Serafini, A.; Lis, M.; Carreras, N.; Boemo, R.L.; Tornero, J. Materials science and engineering C. Biomimetic and supramolecular systems Vol. 33, num. 7, p. 4002-4008 DOI: 10.1016/j.msec.2013.05.034 Date of publication: 2013-10 Journal article
The primary advantages of electrospun membranes include the ability to obtain very thin fibers that are on the order of magnitude of several nanometers with a considerable superficial area and the possibility for these membranes to be manipulated and processed for many different applications. The purpose of this study is to evaluate and quantify the transport mechanisms that control the release of drugs from polymer-based sandwich membranes produced using the electrospinning processes. These electrospun membranes were composed of poly(lactic acid) (PLA) because it is one of the most promising biodegradable polymers due to its mechanical properties, thermoplastic processability and biological properties, such as its biocompatibility and biodegradability. The transport mechanism that controls the drug delivery was evaluated via the release kinetics of a bioactive agent in physiological serum, which was used as a corporal fluid simulation. To describe the delivery process, mathematical models, such as the Power Law, the classical Higuchi equation and an approach to Fick's Second Law were used. Using the applied mathematical models, it is possible to conclude that control over the release of the drug is significantly dependent on the thickness of the membrane rather than the concentration of the drug.