Valera Cano, Enrique Andres
Total activity: 14
Department
Department of Electronic Engineering
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enrique.valeraestudiant.upc.edu
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Scientific and technological production
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1 to 14 of 14 results
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    Immunosensors: concepts and structures for fast and accurate sensing  Open access

     Rodriguez Martinez, Angel; Valera Cano, Enrique Andres
    Date of publication: 2013-05
    Book chapter

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  • Immunosensors based on interdigitated electrodes for the detection and quantification of pesticides in food

     Valera Cano, Enrique Andres; Rodriguez Martinez, Angel
    Date of publication: 2011-12
    Book chapter

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  • Determination of atrazine residues in red wine samples. A conductimetric solution

     Valera Cano, Enrique Andres; Ramón Azcón, Javier; Barranco, Alejandro; Alfaro, Begoña; Sánchez-Baeza, Francisco; Marco Colas, María Pilar; Rodriguez Martinez, Angel
    Food chemistry
    Date of publication: 2010
    Journal article

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    The potential of a conductimetric immunosensor to detect residual amounts of atrazine in a complexv matrix, such as red wine, is evaluated. The immunosensor presented is based on interdigitated μ-electrodes (IDμEs), immunoreagents specifically developed to detect atrazine, and antibodies labelled with gold nanoparticles. Due to the amplification of the conductive signal, produced by the presence of the gold particles, atrazine can be detected using simple and inexpensive direct current (DC) measurements. Then, sensors response is related to the atrazine concentration in the sample. Likewise, the presence of gold particles generates an increase in the intensity of the electric field between the electrodes. The time for assay completion, for 48 samples, was 5 h. Nevertheless, during the first hour (devoted to the incubation assays), the number of samples could be increased without prejudice the assay duration. Sensor responses obtained using red wine samples are compared with results obtained using buffer solutions. As a consequence, the strong matrix effect related to red wine samples has been identified as a non-specific increase of the current intensity through the device. The limits of detection (LODs) obtained are far below the Maximum Residue Level (50 μg kg-1) established by EU for residues of atrazine as herbicide in the wine grapes and other foodstuff products. This opens the door to commercial sensors of simple manipulation, transportable and economics.

  • Fabrication of flexible interdigitated µ-electrodes (FIDµEs) for the development of a conductimetric immunosensor for atrazine detection based on antibodies labelled with gold nanoparticles

     Valera Cano, Enrique Andres; Muñiz, David; Rodriguez Martinez, Angel
    Microelectronic engineering
    Date of publication: 2010
    Journal article

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    The present paper describes a simple and low-cost method for the fabrication of mechanically flexible interdigitated μ-electrodes (FIDμEs) and its application as immunosensor. FIDμEs consist of two coplanar non-passivated interdigitated metallic μ-electrodes supported on a flexible transparent substrate, polyethylene naphthalate (PEN). Bioreagents deposition on PEN substrates becomes possible by depositing SiO2 on the electrodes surface (fingers and inter-digits space). These FIDμEs were successfully applied for the development of a selective conductimetric immunosensor for the quantification of atrazine residues. The immunosensor has been demonstrated for detection of small amounts of atrazine, thanks to the use of immunoreagents specifically developed to detect this pesticide. The detection method applied is based on the use of antibodies labelled with gold nanoparticles. The presence of these particles amplifies the conductive signal; hence the immunosensor response was quantified using simple and inexpensive DC measurements. Immunochemical detection of the concentrations of atrazine is achieved by a competitive reaction which occurs before the inclusion of the labelled antibodies. The immunosensor shows limits of detection in the order of 2–3 μg L−1, far below the maximum residue level (50 μg kg−1) established by EU for residues of atrazine as herbicide in the wine grapes and other foodstuff products.

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    Interdigitated u-electrodes for development of an impedimetric immunosensor for atrazine detection  Open access

     Valera Cano, Enrique Andres; Ramón-Azcón, Javier; Sanchez, F. J.; Marco, M. P.; Rodriguez Martinez, Angel; Castañer Muñoz, Luis Maria
    Spanish Conference on Electron Devices
    Presentation of work at congresses

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    This contribution describes the development of an impedimetric immunosensor for atrazine detection. This immunosensor is based on the use of interdigitated metallic μ-electrodes (IDμEs) The method described in this work does not use any redox mediator and relies on the direct detection of immunochemical competitive reaction between the pesticide and a haptenized-protein immobilized on interdigitated μ-electrodes for the specific antibody. The immunoreagents used were specifically developed to detect atrazine. The immunochemical detection of this pesticide is achieved without using any label. The immunosensor shows a limit of detection of 8.34±1.37 μg L-1, witch is lower than the Maximun Residue Level (MRL) (50μg L-1)established by EU (European Union)for residues of atrazine as herbicide in the wine grapes and other foodstuff products.

  • Immunosensors for atrazine detection in red wine samples

     Valera Cano, Enrique Andres; Rodriguez Martinez, Angel; Azcón, J.R.; Sánchez Baeza, Francisco J.; Marco Colas, María Pilar
    International Conference on Biomedical Electronics and Devices
    Presentation's date: 2009-01
    Presentation of work at congresses

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    Two novel immunosensors, one impedimetric and other one conductimetric, for atrazine detection in red wine samples have been developed. Impedimetric immunosensor is based on an array of interdigitated μ- electrodes (IDμEs) and bioreagents specifically developed to detect this pesticide. Conductimetric immunosensor incorporates additionally gold nanoparticles. Bioreagents were covalently immobilized on the surface of the electrodes (interdigital space). In both cases the biochemical determination of atrazine is possible without any redox mediator. For the case of the impedimetric immunosensor, the detection method is based on impedimetric measurements (in a wide range of frequencies), whereas in the case of the conductimetric immunosensor the detection method is based on conductimetric measurements (DC measurements).The potential of the impedimetric immunosensor to analyze atrazine in complex sample matrices, such as red wine, have been evaluated. This immunosensor can detect atrazine with limits of detection in the order sub-ppb, far below the maximum residue level (MRL) (50 μg L−1) established by European Union (EU) for residues of this herbicide in the wine grapes.

  • Immunosensors based on Interdigitated µ-Electrodes: Application to Pesticide Detection

     Valera Cano, Enrique Andres
    Defense's date: 2008-09-29
    Department of Electronic Engineering, Universitat Politècnica de Catalunya
    Theses

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  • Conductimetric immunosensor for atrazine detection based on antibodies labelled with gold nanoparticles

     Valera Cano, Enrique Andres; Ramon-Azcon, J; Sanchez, F J; Marco, M P; Rodriguez Martinez, Angel
    Sensors and actuators B. Chemical
    Date of publication: 2008-09
    Journal article

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  • Single frequency impedimetric biosensor for atrazine detection

     Rodriguez Martinez, Angel; Valera Cano, Enrique Andres; Ramón-Azcón, J; Sanchez, J; Marco, M P; Castañer Muñoz, Luis Maria
    Sensors and actuators B. Chemical
    Date of publication: 2008-02
    Journal article

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  • An impedimetric immunosensor based on interdigitated microelectrodes (ID mu E) for the determination of atrazine residues in food samples

     Ramon-Azcon, J; Valera Cano, Enrique Andres; Rodriguez Martinez, Angel; Barranco, A; Alfaro, B; Sanchez-Baeza, F; Marco, M P; Castañer Muñoz, Luis Maria
    Biosensors and bioelectronics
    Date of publication: 2008-04
    Journal article

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    Immunosensor impedimétrico para la detección de pesticidas  Open access

     Valera Cano, Enrique Andres; Ramón-Azcon, Javier; Sánchez Baeza, Francisco; Marco Colas, María Pilar; Rodriguez Martinez, Angel
    Congreso Español de Ingeniería de Alimentos y Congreso Iberoamericano sobre Seguridad Alimentaria (CESIA-CIBSA)
    Presentation's date: 2008-11
    Presentation of work at congresses

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    En este trabajo describimos un immunosensor impedimétrico para la detección de pesticidas. Para demostrar dicho sensor hemos utilizado atrazina, como pesticida de test. Este sensor está basado en el uso de μ-electrodos interdigitados así como en reactivos específicamente desarrollados para la detección de este pesticida. Los anticuerpos utilizados no incluyen ningún tipo de etiqueta. Así mismo, el sensor no incluye ningún tipo de par redox que amplifique la señal. La detección immunoquímica de atrazina se alcanza mediante una reacción competitiva entre el antígeno tapizado y el pesticida por una pequeña cantidad de anticuerpo. Los cambios en la impedancia producidos por la inclusión de los bioreactivos son interpretados utilizando un circuito equivalente, el cual representa el sistema de manera fiable. La detección se monitoriza a partir de medidas impedimétricas diferenciales en un amplio espectro de frecuencia. El immunosensor muestra límites de detección en el rango de pocos ppb's, lo cual está muy por debajo del Maximum Residue Level (MRL) (50 μg L-1) establecido por la Unión Europea para los residuos de atrazina en uvas de vino así como en otros productos alimenticios. Aunque en este trabajo el immunosensor se ha demostrado para la atrazina, otros pesticidas podrían detectarse mediante este método siempre que se utilicen los reactivos adecuados.

    Postprint (author’s final draft)

  • Impedimetric immunosensor for atrazine detection using interdigitated mu-electrodes (ID mu E's)

     Valera Cano, Enrique Andres; Ramon-Azcon, J; Rodriguez Martinez, Angel; Castaner, L; Castañer Muñoz, Luis Maria; Sanchez, F J; Marco, M P
    Sensors and actuators B. Chemical
    Date of publication: 2007-08
    Journal article

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  • Steady State and Transient Conductivity of Colloidal Solutions of Gold Nanobeads

     Valera Cano, Enrique Andres; Rodriguez Martinez, Angel; Castaner, L; Castañer Muñoz, Luis Maria
    IEEE transactions on nanotechnology
    Date of publication: 2007-09
    Journal article

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  • Development of microstructured zeolite films as highly accessible catalytic coatings for microreactors

     Urbiztondo, M A; Valera Cano, Enrique Andres; Todorov Trifonov, Trifon; Alcubilla Gonzalez, Ramon; Irusta, S; Pina, M P; Rodriguez Martinez, Angel; Santamaria, J
    Journal of catalysis
    Date of publication: 2007-08
    Journal article

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