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Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the identification of species involved in human infections

Autor
del Valle, LJ.; Jaramillo, M.; Talledo, M.; Pons, M.; Flores, L.; Quispe, R.; Ramírez, P.; García, R.; Alvarado, D.; Espinoza, A.; Del Valle, J.; Vargas, M.; Ruiz, J.
Tipus d'activitat
Article en revista
Revista
Universal Journal of Microbiology Research
Data de publicació
2014
Volum
2
Número
1
Pàgina inicial
15
Pàgina final
22
DOI
10.13189/ujmr.2014.020102
Repositori
http://hdl.handle.net/2117/102572 Obrir en finestra nova
Resum
We designed a 16S rRNA gene PCR -RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were Mae III, Mse I, Sau96 I, Bsa AI, Drd I, Fok I, Bss HII, Bst UI, Alu I, Tsp DTI and Hph I which, according to a decision -making tree, faci litated the differentiation of all the currently described species of Bartonel...
Citació
del Valle, LJ., Jaramillo, M., Talledo, M., Pons, M., Flores, L., Quispe, R., Ramírez, P., García, R., Alvarado, D., Espinoza, A., Del Valle, J., Vargas, M., Ruiz, J. Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the identification of species involved in human infections. "Universal Journal of Microbiology Research", 2014, vol. 2, núm. 1, p. 15-22.
Paraules clau
16s rRNA Gene, Bartonella, Identification, PCR-RFLP
Grup de recerca
CRnE - Centre de Recerca en Ciència i Enginyeria Multiescala de Barcelona
PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables.

Participants

  • Del Valle Mendoza, Luis Javier  (autor)
  • Jaramillo, Michael L.  (autor)
  • Talledo, Miguel  (autor)
  • Pons, Maria J.  (autor)
  • Flores, Lídia  (autor)
  • Quispe, Ruth  (autor)
  • Ramírez, Pablo  (autor)
  • García de la Guarda, Ruth  (autor)
  • Alvarado, Débora  (autor)
  • Espinoza Culupú, Abraham  (autor)
  • Del Valle, Juana  (autor)
  • Vargas, Martha  (autor)
  • Ruiz, Joaquim  (autor)