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False-Positive Viability PCR Results: An Association with Microtubes

Autor
Agusti, G.; Fittipaldi, M.; Codony, F.
Tipus d'activitat
Article en revista
Revista
Current microbiology
Data de publicació
2017-03-01
Volum
74
Número
3
Pàgina inicial
377
Pàgina final
380
DOI
https://doi.org/10.1007/s00284-016-1189-3 Obrir en finestra nova
URL
https://link.springer.com/article/10.1007%2Fs00284-016-1189-3 Obrir en finestra nova
Resum
Currently, one of the most challenged points to expand the use of viability PCR technique is achieving the complete exclusion of dead cells amplification signals, thus avoiding the overestimation of live cells population. Considering that, and based on the hypothesis that DNA may be retained by microtube walls, the impact of the microtube was addressed on signals from live and heat-killed cells. A double-dye reagent, PEMAX™, which comprises a mix of photo-reactive azide forms of phenanthridium...

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