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Real-time bioluminescence imaging of cell distribution, growth, and differentiation in a three-dimensional scaffold under interstitial perfusion for tissue engineering

Autor
Vila, O.; Garrido, C.; Guerra, M.; Navarro, M.; Meca-Cortes, O.; Mas, S.; Engel, E.; Rubio, N.; Blanco, J.
Tipus d'activitat
Article en revista
Revista
Tissue engineering. Part C, methods
Data de publicació
2016-09-01
Volum
22
Número
9
Pàgina inicial
864
Pàgina final
872
DOI
https://doi.org/10.1089/ten.tec.2014.0421 Obrir en finestra nova
Repositori
http://hdl.handle.net/2117/105536 Obrir en finestra nova
Resum
Bioreactor systems allow safe and reproducible production of tissue constructs and functional analysis of cell behavior in biomaterials. However, current procedures for the analysis of tissue generated in biomaterials are destructive. We describe a transparent perfusion system that allows real-time bioluminescence imaging of luciferase expressing cells seeded in scaffolds for the study of cell–biomaterial interactions and bioreactor performance. A prototype provided with a poly(lactic) acid sc...
Citació
Vila, O., Garrido, C., Guerra, M., Navarro, M., Meca-Cortes, O., Mas, S., Engel, E., Rubio, N., Blanco, J. Real-time bioluminescence imaging of cell distribution, growth, and differentiation in a three-dimensional scaffold under interstitial perfusion for tissue engineering. "Tissue engineering. Part C, methods", 1 Setembre 2016, vol. 22, núm. 9, p. 864-872.
Grup de recerca
CREB - Centre de Recerca en Enginyeria Biomedica
IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies

Participants

  • Vila, Olaia  (autor)
  • Garrido, Cristina  (autor)
  • Guerra Rebollo, Marta  (autor)
  • Navarro Toro, Melba  (autor)
  • Meca-Cortes, O  (autor)
  • Mas, S  (autor)
  • Engel Lopez, Elisabet  (autor)
  • Rubio, Nuria  (autor)
  • Blanco, Jeronimo  (autor)