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Immobilisation of horseradish peroxidase on Eupergit®C for the enzymatic elimination of phenol

Autor
Pramparo, L.; Stüber, F.; Font, J.; Fortuny, A.; Fabregat, A.; Bengoa, C.
Tipus d'activitat
Article en revista
Revista
Journal of hazardous materials
Data de publicació
2010-05-15
Volum
177
Número
1-3
Pàgina inicial
990
Pàgina final
1000
DOI
https://doi.org/10.1016/j.jhazmat.2010.01.017 Obrir en finestra nova
Repositori
http://hdl.handle.net/2117/6675 Obrir en finestra nova
Resum
In this study, three different approaches for the covalent immobilisation of the horseradish peroxidase (HRP) onto epoxy-activated acrylic polymers (Eupergit®C) were explored for the first time, direct HRP binding to the polymers via their oxirane groups, HRP binding to the polymers via a spacer made from adipic dihydrazide, and HRP binding to hydrazido polymer surfaces through the enzyme carbohydrate moiety previously modified by periodate oxidation. The periodate-mediated covalent immobilisat...
Citació
Pramparo, L. [et al.]. Immobilisation of horseradish peroxidase on Eupergit®C for the enzymatic elimination of phenol. "Journal of hazardous materials", 15 Maig 2010, vol. 177, núm. 1-3, p. 990-1000.

Participants

  • Pramparo, Laura  (autor)
  • Stüber, Frank Erich  (autor)
  • Font Capafons, Josep  (autor)
  • Fortuny Sanroma, Agustin  (autor)
  • Fabregat Llagostera, Azael  (autor)
  • Bengoa, Christophe José  (autor)