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Viable real-time PCR in environmental samples: can all data be interpreted directly?

Autor
Fittipaldi, M.; Codony, F.; Adrados, B.; Camper, A.; Morato, J.
Tipus d'activitat
Article en revista
Revista
Microbial ecology
Data de publicació
2010-06-25
Volum
61
Número
1
Pàgina inicial
7
Pàgina final
12
DOI
https://doi.org/10.1007/s00248-010-9719-1 Obrir en finestra nova
Repositori
http://hdl.handle.net/2117/10870 Obrir en finestra nova
URL
http://www.springerlink.com/content/h58rtt2115h73377/ Obrir en finestra nova
Resum
Selective nucleic acid intercalating dyes—ethidium monoazide (EMA) and propidium monoazide (PMA)— represent one of the most successful recent approaches to detect viable cells (as defined by an intact cell membrane) by PCR and have been effectively evaluated in different microorganisms. However, some practical limitations were found, especially in environmental samples. The aim of this work was to show that in the application of viable real-time PCR, there may be significant biases and to pr...
Citació
Fittipaldi, M. [et al.]. Viable real-time PCR in environmental samples: can all data be interpreted directly?. "Microbial ecology", 25 Juny 2010.
Grup de recerca
CEBIM - Centre de Biotecnologia Molecular
TMAS (T+) - Toxicologia i Microbiologia Ambiental i Sanitària

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