Engel, E.; Serrano, S.; Mariñoso, M.; Lloreta, J.; Ulloa, F.; Nogués, X.; Díez-Perez, A.; Carbonell, A. Calcified tissue international Vol. 3, num. 72, p. 228-235 Data de publicació: 2003-03 Article en revista
Engel, E.; Serrano, S.; Mariñoso, M.; Lloreta, J.; Ulloa, F.; Nogués, X.; Díez-Perez, A.; Carbonell, J. Calcified tissue international Vol. 72, num. 3, p. 228-235 Data de publicació: 2003 Article en revista
Bisphosphonates exert a potent inhibitory
effect on bone resorption. Several studies have been performed, with contradictory results, to ascertain whether the effect of bisphosphonates on osteoclasts could be produced, at least in part, by modulation of the synthesis of resorption-promoting factors by osteoblasts.
The aim of this study was to evaluate the effect of etidronate (10)4–10)9 M) and alendronate (10)7–10)12 M) on the production of IL-6 and IL-11 using human osteoblast cultures. Cytokines were quantified by ELISA,
and mRNA expression was tested. Treatment with alendronate and etidronate had no effect on the synthesis of IL-6 or IL-11, and IL-6 and IL-11 mRNA levels. These results were obtained both in nonstimulated
cultures and in cultures stimulated by means of TNF-a, IL-1b, and TNF-a+IL-1b, with or without FCS. In conclusion, a possible indirect osteoclast-mediated effect of alendronate and etidronate on bone resorption
would not be exerted through reduction in
osteoblastic synthesis of IL-6 and IL-11.