Cano, O.; Céspedes, M. V.; Unzueta, U.; Roldan, M.; Cubarsi, R.; Vazquez, E.; García, E.; Villaverde, A. Nanomedicine Vol. 18, num. 18, p. 2387-2398 DOI: 10.2217/nnm-2016-0200 Data de publicació: 2016-09-01 Article en revista
Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-assembling protein materials. Materials & methods: Biophysical properties, cell penetrability and in vivo biodistribution upon systemic administration of tumor-targeted protein nanoparticles produced in L. lactis have been compared with the equivalent material produced in E. coli. Results: Protein nanoparticles have been efficiently produced in L. lactis, showing the desired size, internalization properties and biodistribution. Conclusion: In vitro and in vivo data confirm the potential and robustness of the production platform, pointing out L. lactis as a fascinating cell factory for the biofabrication of protein materials intended for therapeutic applications.