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Discrimination of viable "acanthamoeba castellani" trophozoites and cysts by propidium monoazide real-time polymerase chain reaction

Autor
Fittipaldi, M.; Pino, N.; Adrados, B.; Agusti, G.; Peñuela, G.; Morato, J.; Codony, F.
Tipus d'activitat
Article en revista
Revista
Journal of eukaryotic microbiology
Data de publicació
2011-08
Volum
58
Número
4
Pàgina inicial
359
Pàgina final
364
DOI
https://doi.org/10.1111/j.1550-7408.2011.00557.x Obrir en finestra nova
Repositori
http://hdl.handle.net/2117/13331 Obrir en finestra nova
Resum
Even though the advent of quantitative polymerase chain reaction (PCR) has improved the detection of pathogen microorganisms in most of areas of microbiology, a serious limitation of this method may arise from the inability to discriminate between viable and nonviable pathogens. To overcome it, the use of real-time PCR and selective nucleic acid intercalating dyes like propidium monoazide (PMA) have been effectively evaluated for different microorganisms. To assess whether PMA pretreatment can i...
Citació
Fittipaldi, M. [et al.]. Discrimination of viable acanthamoeba castellani trophozoites and cysts by propidium monoazide real-time polymerase chain reaction. "Journal of eukaryotic microbiology", Agost 2011, vol. 58, núm. 4, p. 359-364.
Grup de recerca
CEBIM - Centre de Biotecnologia Molecular
SUMMLab - Sustainability Measurement and Modeling Lab
TMAS (T+) - Toxicologia i Microbiologia Ambiental i Sanitària

Participants