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Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy

Autor
Aviles, R.; Andilla, J.; Porcar-Guezenec, R.; Olarte, O.; Nieto, M.; Levecq, X.; Artigas, D.; Loza, P.
Tipus d'activitat
Article en revista
Revista
Biomedical optics express
Data de publicació
2011-11-01
Volum
2
Número
11
Pàgina inicial
3135
Pàgina final
3149
DOI
https://doi.org/10.1364/BOE.2.003135 Obrir en finestra nova
Repositori
http://hdl.handle.net/2117/16402 Obrir en finestra nova
URL
http://www.ncbi.nlm.nih.gov/pubmed/22076274 Obrir en finestra nova
Resum
We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear guide-star (NL-GS). The wavefront emitted from the NL-GS can then be recorded using a Shack-Hartmann sensor. Compensation of the recorded sample aberrations is performed by the deformable mirror in a single-step. This technique is applied to fixed and in vivo biological sample...
Citació
Aviles, R. [et al.]. Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy. "BIOMEDICAL OPTICS EXPRESS", 01 Novembre 2011, vol. 2, núm. 11, p. 3135-3149.
Paraules clau
ADAPTIVE OPTICS, DEEP-TISSUE, EXCITATION, IMPLEMENTATION, MULTIPHOTON MICROSCOPY, OPTIMIZATION, SPHERICAL-ABERRATION
Grup de recerca
FOTONICA - Grup de Recerca de Fotònica

Participants

  • Aviles Espinosa, Rodrigo  (autor)
  • Andilla, Jordi  (autor)
  • Porcar-Guezenec, Rafael  (autor)
  • Olarte, Omar Eduardo  (autor)
  • Nieto, Marta  (autor)
  • Levecq, Xavier  (autor)
  • Artigas Garcia, David  (autor)
  • Loza Alvarez, Pablo  (autor)