'Hospital-acquired (nosocomial) infections are a major financial issue in the European healthcare system. The financial impact of these infections counteract medical advances and expensive medical treatments by increasing the length of hospital stay by at least 8 days on average per affected patient, hence adding more than 10 millions patient days in hospitals in Europe per year. The statistics on patient safety in the EU show alarming tendencies : - 1 in 10 patients are affected by hospital-acquired infections - 3 million deaths are caused by hospital-acquired infections An active infection control program of patients and personnel and hygiene measures, have proven to significantly reduce both the number of infections and hospitalisation costs . The SONO project directly addresses the above problems by developing a pilot line for the production of medical antibacterial textiles. The pilot line will be based on the scale-up of a sonochemical process developed and patented at BIU laboratories. The pilot line will use a sonochemical technique to produce and deposit inorganic, antimicrobial nanoparticles on medical textiles, e.g. hospital sheets, medical coats and bandages. Sonicators are used industrially for heavy and light duty cleaning, for water disinfection and for sewage treatment. It is also used in the food industry for emulsification and drying. The proposed concept based on one step sonochemical process to produce nanoparticles and impregnate them as antibacterial factors on textile is novel and does not exist on an industrial scale. The concept has already been proven (and patented ) on a lab scale where sonochemistry was applied to impregnate nanoparticles in a single-step process. It was demonstrated that due to the special properties of the sonochemical method the antibacterial nanoparticles are adsorbed permanently on the fibres even after 70 “laundry cycles”. The sonochemical impregnation process is a one-step procedure in which the nanopa'
Calafell, M.; Diaz, C.; Hadzhiyska, H.; Gibert, J.; Daga, J.; Tzanov, T. Biocatalysis and biotransformation Vol. 25, num. 2-4, p. 336-340 DOI: 10.1080/10242420701379874 Data de publicació: 2007-03 Article en revista
Cotton fabrics were dyed with dyes generated in situ by laccase-catalyzed oxidative coupling of the colorless 2,5-diaminobenzenesulfonic acid (2,5-DABSA) and 1-hydroxyphenol (catechol). The enzymatic oxidation of the dye intermediates led to cross-coupling reaction products when the reaction was conducted with an excess of catechol. At least fourfold excess of catechol was necessary to achieve satisfactory dye fixation on cotton. Formation of the same colored product using either an equimolar ratio of the reagents or tenfold excess of catechol was observed. Most probably, homo-molecular reactions predominate over the cross-coupling at equimolar ratio of the precursors, while with an excess of catechol, the cross-coupling occurs in higher yield. The reaction was followed using UV-Vis spectroscopy, HPLC, FTIR and MALDI-TOF MS. A reaction pathway for laccase-induced cross-coupling of catechol and 2,5-DABSA yielding a major colored product was proposed.