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A new spiral microelectrode assembly for electroporation and impedance measurements of adherent cell monolayers

Autor
García-Sánchez, T.; Guitart, M.; Rosell, F.; Gomez; Bragos, R.
Tipus d'activitat
Article en revista
Revista
Biomedical microdevices
Data de publicació
2014-08-01
Volum
16
Número
4
Pàgina inicial
575
Pàgina final
590
DOI
https://doi.org/10.1007/s10544-014-9860-6 Obrir en finestra nova
Repositori
http://hdl.handle.net/2117/25348 Obrir en finestra nova
Resum
In this study, a new microelectrode assembly based on spiral geometry applicable to in situ electroporation of adherent cell monolayers on standard multiwell plates is presented. Furthermore, the structure is specially conceived to perform electrical impedance spectroscopy (EIS) measurements during electroporation. Its performance for cell membrane permeabilization is tested with a fluorescent probe. Gene electrotransfer is also assayed using a plasmid DNA encoding GFP in four different cell lin...
Citació
García-Sánchez, T. [et al.]. A new spiral microelectrode assembly for electroporation and impedance measurements of adherent cell monolayers. "Biomedical microdevices", 01 Agost 2014, vol. 16, núm. 4, p. 575-590.
Paraules clau
Adherent cell, DNA ELECTROTRANSFER, ELECTRIC PULSES, ELECTROPERMEABILIZATION, ELECTROTRANSFER IN-VITRO, EXPRESSION, Electrical impedance spectroscopy, GENE-TRANSFER, Gene electrotransfer, In situ electroporation, LOW-VOLTAGE PULSES, MAMMALIAN-CELL, Microelectrodes, SITU ELECTROPORATION, TISSUE ELECTROPORATION, siRNA delivery
Grup de recerca
CREB - Centre de Recerca en Enginyeria Biomedica
IEB - Instrumentació Electrònica i Biomèdica

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