CytoNanoHeal aims at engineering of delivery systems that enable long-term efficiency of biological entities in cell-based therapies. The cell-matched rationale of the novel injectable supramolecular nanofibrous hydrogels based on self-assembling peptide amphiphiles, complemented with glycosaminoglycan nanoparticle (GAG-NPs) templates, will allow long-term efficiency of various biological therapies. As a proof-of-concept in regenerative medicine, the nanoconstructs will be used to provide optimal spatial and functional support for bioentities in a combined therapy for osteoarthritis (OA) comprising: - Cytokines for retarding the disease and pain relief, and - Stem cells for cartilage regeneration. A patent-protected anti-inflammatory cytokine cocktail (ACC), formulated from human blood plasma will be injected together with in-situ gelating peptide amphiphiles and GAG-NPs into the OAaffected joints to delay the disease progression and boost the anabolic pathways for recovery of the damaged cartilage. The ACC is a synergistic combination of bioentities that inhibit the chronicity of OA pro-inflammatory factors. The novel delivery systems will be used to protect the unstable cytokines and boost the therapeutic effect. Additionally, mesenchymal stem cells from adipose tissue (ASCs) will be supplied using the engineered nanoconstructs to initiate the cartilage regeneration by differentiation of ASCs into chondrocytes. UPC will play the role as coordinator of the European project. UPC is the last responsible for managing CytoNanoHeal by monitoring the scientific, financial and the dissemination activities of the results of the consortium. UPC will also be responsible for the tasks required to fulfill the following specific objectives. - Development and characterization of GAG-NPs templates to improve the efficiency of the ACC cocktails and stimulate the cellular interactions promoting the cartilage regeneration. - Characterize the injectable supramolecular hydrogels efficiency in order to stabilize ASC and other, capable of housing several biological entities. - Study the efficacy vs. toxicity balance and the biocompatibility of the constructs.
Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016
Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad