Paper is a natural, renewable and biodegradable product which shows a high versatility, what makes it feasible the proposal of creating a low cost and portable microdevice for immediate biodiagnosis. Besides, according to OMS these systems for health surveillance should accomplish several criteria, several of which are not solved yet for their industrial application. The present projects plans the design of several paper based components, that fulfil the different needs of analytical methods, and that could be integrated together to produce a single 3D microdevice for biodiagnosis, showing multiple functionality adapted to each of the analysis purposes. For this objective there is the need of new technologies (Biotechnology, Nanotechnology and Micro and Nanoelectronics). Microfluidics will allow the transport and efficient mixing of the samples, while analysis response sensitivity will be enhanced by printed microelectronics. More relevant aspects are: a) application of new enzymatic technologies for the modification and functionalization of cellulose; b) analysis of biodegradability and life cycle of the microdevice; c) expand the incidence of the new device to countries under development and of low income; d) great repercussion of the microdevice in health care systems; e) application of transversal technologies: advanced materials, biotechnology, nanotechnology and micro and nanoelectronics The proposed objectives are: 1) Identification and development of new cellulose modifying enzymes: lytic polysaccharide monooxigenases (LPMO), expansins and new cellulases; 2) Hydrophobic functionalization of nanofibrilar cellulose (NFC) by enzyme technology; 3) Designing of porous platforms from different cellulosic fibers to create a microfluidic circuit; 4) Application of bacterial cellulose (CB) in paper products; 5) Production of cellulose hydrogels by sustainable methods; 6) Production of cellulosic support with surface smoothness at nanoscale, and hydrophobic to print conducting inks and design electronic microcircuits; 7) Designing of sample transfer system among different cellulosic supports; 8) Production of antioxidant oligosaccharides from hemicellulose; 9) Designing of optical and/or electronic detection systems of immediate reading and easy interpretation, and personalized for the analysis aims; 10) Create a 3D biodiagnosis microdevice that is robust, portable, easy to use and biodegradable; 11) Evaluate biodegradability and life cycle of the produced microdevices The expected results are: a) Identification and designing of new enzymes for the increase of paper mechanical properties; b) Designing of cellulosic supports with different properties from the mixing of different cellulosic or nanocellulosic (vegetal or bacterial) sources; c) Definition of the best combination of raw materials that allows the production of a cellulosic microfluidic platform with high efficiency for liquid transport; d) Development of a device for highly sensitive electronic detection by printing of conducting inks; e) Production of a 3D biodiagnosis device with multiple functionalities, robust, portable, biodegradable and easy to use, for immediate biodiagnosis from natural and renewable resources.
Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016
Programa Estatal de I+D+i Orientada a los Retos de la Sociedad
Vidal, T.; Torres, A.L.; Valls, C.; Cusola, O.; Casals-Terré, J.; Farré-Lladós, J.; Pastor Blasco, Francisco Ignacio Javier; Martinez, J.; Roncero, M.B. American Chemical Society National Meeting & Exposition p. 223- Presentation's date: 2019-04-01 Presentation of work at congresses
Valls, C.; Pastor Blasco, Francisco Ignacio Javier; Vidal, T.; Roncero, M.B.; Diaz Lucea, M. Pilar; Martinez, J.; Valenzuela, S.V. American Chemical Society National Meeting & Exposition p. 116- Presentation's date: 2019-03-31 Presentation of work at congresses
Soder, J.; Valls, C.; Pastor Blasco, Francisco Ignacio Javier; Valeria, S. Congreso Nacional de Microbiología Industrial y Biotecnología Microbiana p. 109 Presentation's date: 2018-06-06 Presentation of work at congresses
Cabañas, L.; Valls, C.; Roncero, M.B.; Fernández, J.; Diaz Lucea, M. Pilar; Martinez, J. Congreso Nacional de Microbiología Industrial y Biotecnología Microbiana p. 89 Presentation's date: 2018-06-06 Presentation of work at congresses