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Validation and implementation of a diagnostic algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B-holmesii in a Pediatric Referral Hospital in Barcelona, Spain

Author
Valero , A.; Henares, D.; Acosta, L.; Jané, M.; Godoy, P.; Muñoz, C.
Type of activity
Journal article
Journal
Journal of clinical microbiology
Date of publication
2019-01-01
Volume
57
Number
1
First page
e01231-1
Last page
e01231-8
DOI
https://doi.org/10.1128/JCM.01231-18 Open in new window
Repository
http://hdl.handle.net/2117/129052 Open in new window
URL
https://jcm.asm.org/content/57/1/e01231-18 Open in new window
Abstract
This study aimed to validate a comprehensive diagnostic protocolbased on real-time PCR for the rapid detection and identification ofBordetella per-tussis,Bordetella parapertussis, andBordetella holmesii, as well as its implementationin the diagnostic routine of a reference children’s hospital. The new algorithm in-cluded a triplex quantitative PCR (qPCR) targeting IS481gene (inB. pertussis,B. hol-mesii, and someBordetella bronchisepticastrains), pIS1001(B. parapertussis-specific)andrnaseP as t...
Citation
Valero , A. [et al.]. Validation and implementation of a diagnostic algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B-holmesii in a Pediatric Referral Hospital in Barcelona, Spain. "Journal of clinical microbiology", 1 Gener 2019, vol. 57, núm. 1, p. e01231-1-e01231-8.
Keywords
B. holmesii, B. parapertussis, Bordetella pertussis, real-time PCR, whooping cough
Group of research
ADBD - Analysis of Complex Data for Business Decisions

Participants

  • Valero Rello, Ana  (author)
  • Henares Bonilla, Desirée  (author)
  • Acosta Argueta, Lesly Maria  (author)
  • Jané Checa, Mireia  (author)
  • Godoy Garcia, Pere  (author)
  • Muñoz Almagro, Carmen  (author)

Attachments