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Discrimination of viable "acanthamoeba castellani" trophozoites and cysts by propidium monoazide real-time polymerase chain reaction

Author
Fittipaldi, M.; Pino, N.; Adrados, B.; Agusti, G.; Peñuela, G.; Morato, J.; Codony, F.
Type of activity
Journal article
Journal
Journal of eukaryotic microbiology
Date of publication
2011-08
Volume
58
Number
4
First page
359
Last page
364
DOI
https://doi.org/10.1111/j.1550-7408.2011.00557.x Open in new window
Repository
http://hdl.handle.net/2117/13331 Open in new window
Abstract
Even though the advent of quantitative polymerase chain reaction (PCR) has improved the detection of pathogen microorganisms in most of areas of microbiology, a serious limitation of this method may arise from the inability to discriminate between viable and nonviable pathogens. To overcome it, the use of real-time PCR and selective nucleic acid intercalating dyes like propidium monoazide (PMA) have been effectively evaluated for different microorganisms. To assess whether PMA pretreatment can i...
Citation
Fittipaldi, M. [et al.]. Discrimination of viable acanthamoeba castellani trophozoites and cysts by propidium monoazide real-time polymerase chain reaction. "Journal of eukaryotic microbiology", Agost 2011, vol. 58, núm. 4, p. 359-364.
Group of research
CEBIM - Molecular Biotechnology Centre
SUMMLab - Sustainability Measurement and Modelin Lab
TMAS (T+) - Environmental and Health Toxicology and Microbiology

Participants